In this module, we have seen that many enzymes can be applied in biocatalytic retrosynthetic analysis, and it is important that this is applied more often in route design, combining bio and chemo-catalysts to design new routes to target molecules. To achieve this, there are some remaining challenges in the field, including the identification of reactions not available in the enzyme tool box and the design of new biocatalysts with broad substrate scope, that are highly active and stable under chemical process conditions, via the combination of enzyme engineering with bioinformatic methods. The ACS GCI PR have created a simple Biocatalysis Guide which shows the more generic transformations that are available and can be factored into retrosynthetic analysis.
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